Natural Hippophae Rhamnoides Yellow with High Quality

• CAS No.: 90106-68-6
• Resource: Natural
• Transport Package: Paper
• Specification: large
• Trademark: china
• Origin: China
• HS Code: 2930400000
• Production Capacity: 5000kg/Year

Product Description

New Phytologist

 

• Sea buckthorn (Hippophae rhamnoides), a horticulturally multipurpose species in the family Elaeagnaceae, can build associations with Frankia actinomycetes to enable symbiotic nitrogen-fixing. Currently, no high-quality reference genome is available for an actinorhizal plant, which greatly hinders the study of actinorhizal symbiotic nodulation.
• Here, by combining short-read, long-read and Hi-C sequencing technologies, we generated a chromosome-level reference genome of H. rhamnoides (scaffold N50: 65Mb, and genome size: 730Mb) and predicted 30812 protein-coding genes mainly on 12 pseudochromosomes.
• Hippophae rhamnoides was found to share a high proportion of symbiotic nodulation genes with Medicago truncatula, implying a shared molecular mechanism between actinorhizal and rhizobial symbioses.
• Phylogenetic analysis clustered the three paralogous NODULE INCEPTION (NIN) genes of H. rhamnoides with those of other nodulating species, forming the NIN group that most likely evolved from the ancestral NLP group. The genome of H. rhamnoides will help us to decipher the underlying genetic programming of actinorhizal symbiosis, and our high-quality genome and transcriptomic resources will make H. rhamnoides a new excellent model plant for actinorhizal symbiosis research.

 

 

Introduction

Root nodule symbiosis (RNS) is an important evolutionary adaptation that enables plants to acquire nitrogen directly from the atmosphere. By far the most widely studied type of RNS is rhizobia-induced symbiosis in legume species (e.g. Lotus japonicus and Medicago truncatula) and in the nonlegume Parasponia (van Velzen etal., 2018; Roy etal., 2020). However, RNS is not limited to these plants. Actinorhizal symbiosis is another widespread plant-microorganism association that leads to the formation of nitrogen-fixing root nodules in a range of actinorhizal plants (Svistoonoff etal., 2014). Among plant symbiotic actinomycetes, Frankia is a typical microbe that is widely associated with >200 eudicots across three orders and eight families (Svistoonoff etal., 2014). Recently, several progresses have been made in actinorhizal symbiosis research through genetic transformation, transcriptomic analysis or other methods in some typical actinorhizal plants, such as Hippophae rhamnoides, Datisca glomerata and Casuarina glauca (Diouf etal., 1995; Berry etal., 2004; Sriskandarajah etal., 2014; Chaudhary & Sharma, 2015). However, compared with well-studied nodulation symbiosis in legumes, the limited availability of chromosome-level reference genomes for many actinorhizal plants restricts further research on actinorhizal nodulation.