Creatine is an amino acid derivative naturally produced by vertebrate animals and predominantly found in muscle cells, holds significant importance in energy storage. It undergoes phosphorylation to form creatine phosphate, which acts as a phosphate donor during the conversion of ADP to ATP, providing the energy required for muscle contraction. Dietary supplementation with creatine has shown potential in improving muscle wasting linked to chronic diseases, including cancer. Within the muscles, a portion of creatine combines with phosphate to generate creatine phosphate, facilitated by the enzyme creatine kinase. This process yields phosphocreatine (PCr), which binds with adenosine diphosphate (ADP) to regenerate ATP, a crucial cellular energy source utilized for short-term energy demands before oxidative phosphorylation takes place. Supplemental creatine exhibits energy-generating effects during anaerobic exercise and may offer neuroprotective and cardioprotective benefits. The interplay between creatine, creatine kinase, and phosphocreatine constitutes a sophisticated cellular energy buffering and transport system, bridging energy production sites in mitochondria with energy consumption sites. Creatine kinase serves as a pivotal enzyme in maintaining cellular energy homeostasis, catalyzing the reversible transfer of the high-energy phosphate bond from PCr to ADP to generate ATP, and reciprocally transferring the high-energy phosphate bond from ATP to creatine to regenerate PCr. During intense exercise and skeletal muscle contraction, the bioenergetic metabolism shifts from predominantly relying on oxidative phosphorylation to favoring anaerobic glycolysis as the primary pathway for ATP production.